How To Make Liquid Culture

Making a liquid culture for mushrooms involves creating a nutrient-rich solution to promote the growth of mycelium. Here are the steps to create a basic liquid culture:

  1. Prepare your equipment: You will need a sterilized glass jar or flask, a sterilized lid or stopper with a small hole, and a pressure cooker or autoclave for sterilization.

  2. Prepare the nutrient solution: Mix together distilled or sterilized water with a nutrient source such as malt extract, potato dextrose, or agar. The ratio of nutrient to water will depend on the specific recipe you are following.

  3. Sterilize the solution: Pour the nutrient solution into the sterilized jar or flask and seal it with the sterilized lid or stopper. Place the container in a pressure cooker or autoclave and heat it to 15 psi for 15-20 minutes to sterilize the solution.

  4. Inoculate the solution: Once the solution has cooled, add a small amount of mycelium to the jar or flask. This can be done by transferring a small piece of colonized agar or by injecting spores into the solution using a sterilized syringe.

  5. Incubate the culture: Place the inoculated liquid culture in a warm, dark place and allow it to incubate. Depending on the species of mushroom, the culture may take anywhere from a few days to a few weeks to fully colonize.

  6. Use the liquid culture: Once the liquid culture has fully colonized, it can be used to inoculate other substrates such as grain or sawdust to create mushroom spawn.


We have an assortment of All-In-One Mushroom Grow Bags aka "Fun Bags' for your growing pleasure. Fun bags are literally a window into how mother nature produces mushrooms right inside the bag. 

Our fun bags are a great family or school project. Teach kids how to grow their own mushrooms and they will never go hungry. No yard needed. Grow right indoors from beginning until harvest. Bags can also be grown outdoors. 

These bags to not contain spores or liquid culture. Inoculate with your favorite mushroom spores or liquid culture. 

If you have any questions or need advice please contact us.