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Wood Mushroom Grow Bags D.I.Y Kit (Yield: 12+ lb of Substrate)

Wood Mushroom Grow Bags D.I.Y Kit (Yield: 12+ lb of Substrate)

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We created this complete kit for those who want to make their own wood based mushroom grow bags at home.

Each Kit includes:

  • 5 pounds of 100% Oak Pellets
  • 1/4 lb of gypsum powder
  • 3 Mushroom Grow Bags

Materials and Equipment:

  • Shroomability Hardwood Wood Pellets:, Always use hardwood pellets as softwood pellets may contain substances harmful to mushrooms including glue.
  • Water
  • Gypsum Powder: Helps with substrate moisture retention.
  • Large Container: A vessel or a plastic bag suitable for holding the substrate.
  • Thermometer: To monitor temperature.
  • Heat Source: Stove, hot water bath, pressure cooker or other heating methods.


  • Wood Pellets: Use hardwood pellets for the substrate.
  • Hydrating the Wood Pellets:
    • Place the wood pellets in a large container.
    • Add boiling water with gypsum powder dissolve to fully saturate the pellets. Stir well for a few minutes.
  • Pack the Substrate:
    • Pack the hot substrate into bags or containers suitable for pasteurization.
  • Pasteurization:
    • There are several methods for pasteurizing the substrate. Here are two common methods:
    • a. Hot Water Bath: Immerse the bags or containers in hot water (around 160-180°F for 2 hours. Maintain the temperature within this range.
    • b. Steam Pasteurization: Use a steam sterilizer or pressure cooker. Steam the substrate at around 160-180°F for 2 hours.
    • c: Pressure Cook: at 10 PSI for 1 hour.
  • Cooling:
    • Allow the pasteurized substrate to cool to room temperature before inoculating with mushroom spores or mycelium.
  • Inoculation:
    • After cooling, inoculate the substrate with mushroom spores or mycelium culture in a clean, sterile environment.
  • Incubation:
    • Place the inoculated substrate in a warm, dark place for the mycelium to colonize the substrate.

Remember to maintain cleanliness and use proper sterile techniques throughout the process to prevent contamination. Adjustments to the specific conditions may be necessary based on the mushroom species you are cultivating. Always refer to specific guidelines for the type of mushroom you're growing for the best results.

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